Genetic differentiation of trout (Salmo spp.) populations in Serbia ascertained using RFLP technique on PCR amplified control region of mitochondrial DNA

Abstract

Genetic variability of trout populations from 13 localities situated in all three sea basins (Black, Aegean and Adriatic) of Serbia was ascertained using the restriction endonuclease Alu I, i.e., by RFLP (Restriction Fagment Length Polymorphism) technique on PCR (Polymerase Chain Reaction) amplified control region of the mtDNA. Restriction endonuclease Alu I cut the control region of the mtDNA at two characteristic profiles featured by populations of trout from the the Black Sea basin and those from basins of Aegean and Adriatic Seas ("southern" populations), respectively. This revealed a strong correlation between the geographic situation and the genetic differentiation of trout populations.