Please use this identifier to cite or link to this item:
https://biore.bio.bg.ac.rs/handle/123456789/1223
DC Field | Value | Language |
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dc.contributor.author | Dragicevic, Ana | en_US |
dc.contributor.author | Dzopalic, Tanja | en_US |
dc.contributor.author | Vasilijic, Sasa | en_US |
dc.contributor.author | Vucevic, Dragana | en_US |
dc.contributor.author | Tomic, Sergej | en_US |
dc.contributor.author | Božić, Biljana | en_US |
dc.contributor.author | Colic, Miodrag | en_US |
dc.date.accessioned | 2019-09-09T08:51:37Z | - |
dc.date.available | 2019-09-09T08:51:37Z | - |
dc.date.issued | 2012-01-01 | - |
dc.identifier.issn | 1465-3249 | - |
dc.identifier.uri | https://biore.bio.bg.ac.rs/handle/123456789/1223 | - |
dc.description.abstract | Background aims. Recent studies have shown that the ligation of Toll-like receptor 3 (TLR3) or Dectin-1 on human monocyte-derived dendritic cells (MoDC) elicits their maturation, but with a different outcome on immunomodulation. Therefore the aim of this work was to study the response of MoDC to the combined effect of polyinosinic:polycytydilic acid [Poly (I:C)] and curdlan, selective TLR3 and Dectin-1 agonists, respectively. Methods. Immature MoDC, generated from human monocytes, were treated with Poly (I:C), curdlan or their combination for 2 days. Phenotypic characteristics of MoDC were determined by flow cytometry, and cytokine production was measured by enzyme-linked immunosorbent assay (ELISA) and FlowCytomix, while the stimulatory capability of MoDC was tested using a mixed leukocyte reaction assay. Results. The combination of Poly (I:C) and curdlan induced phenotypic maturation of MoDC with the capability to stimulate an alloreactive response. Such treated MoDC up-regulated the production of interleukin (IL)-12, IL-23 and IL-10, compared with the effect of Poly (I:C) alone. Curdlan-treated MoDC stimulated the production of IL-17 by alloreactive CD4 + T cells more strongly than Poly (I:C)-treated MoDC. The opposite effect was observed for interferon(IFN)-γ production. When combined, these agonists primed MoDC to increase further the production of IFN-γ by CD4 + T cells in co-culture, especially those of naive (CD45RA +) phenotype, and IL-17 by memory (CD45RO +) CD4 + T cells. Conclusions. Ligation of TLR3 and Dectin-1 receptor up-regulates T-helper (Th) 1 and Th17 immune responses compared with single agonists. These findings may have therapeutic implications for the use of MoDC in immunotherapy. © 2012 Informa Healthcare. | en_US |
dc.language.iso | en | en_US |
dc.relation.ispartof | Cytotherapy | en_US |
dc.subject | Dectin-1 receptor | en_US |
dc.subject | Human monocyte-derived dendritic cells | en_US |
dc.subject | T-helper immune response | en_US |
dc.subject | Toll-like receptor 3 | en_US |
dc.title | Signaling through Toll-like receptor 3 and Dectin-1 potentiates the capability of human monocyte-derived dendritic cells to promote T-helper 1 and T-helper 17 immune responses | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.3109/14653249.2012.667873 | - |
dc.identifier.pmid | 22424215 | - |
dc.identifier.scopus | 2-s2.0-84859745341 | - |
dc.identifier.url | https://api.elsevier.com/content/abstract/scopus_id/84859745341 | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | Article | - |
item.fulltext | With Fulltext | - |
item.grantfulltext | restricted | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.author.dept | Chair of General Physiology and Biophysics | - |
crisitem.author.orcid | 0000-0002-1238-1731 | - |
Appears in Collections: | Journal Article |
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