Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/1223
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dc.contributor.authorDragicevic, Anaen_US
dc.contributor.authorDzopalic, Tanjaen_US
dc.contributor.authorVasilijic, Sasaen_US
dc.contributor.authorVucevic, Draganaen_US
dc.contributor.authorTomic, Sergejen_US
dc.contributor.authorBožić, Biljanaen_US
dc.contributor.authorColic, Miodragen_US
dc.date.accessioned2019-09-09T08:51:37Z-
dc.date.available2019-09-09T08:51:37Z-
dc.date.issued2012-01-01-
dc.identifier.issn1465-3249-
dc.identifier.urihttps://biore.bio.bg.ac.rs/handle/123456789/1223-
dc.description.abstractBackground aims. Recent studies have shown that the ligation of Toll-like receptor 3 (TLR3) or Dectin-1 on human monocyte-derived dendritic cells (MoDC) elicits their maturation, but with a different outcome on immunomodulation. Therefore the aim of this work was to study the response of MoDC to the combined effect of polyinosinic:polycytydilic acid [Poly (I:C)] and curdlan, selective TLR3 and Dectin-1 agonists, respectively. Methods. Immature MoDC, generated from human monocytes, were treated with Poly (I:C), curdlan or their combination for 2 days. Phenotypic characteristics of MoDC were determined by flow cytometry, and cytokine production was measured by enzyme-linked immunosorbent assay (ELISA) and FlowCytomix, while the stimulatory capability of MoDC was tested using a mixed leukocyte reaction assay. Results. The combination of Poly (I:C) and curdlan induced phenotypic maturation of MoDC with the capability to stimulate an alloreactive response. Such treated MoDC up-regulated the production of interleukin (IL)-12, IL-23 and IL-10, compared with the effect of Poly (I:C) alone. Curdlan-treated MoDC stimulated the production of IL-17 by alloreactive CD4 + T cells more strongly than Poly (I:C)-treated MoDC. The opposite effect was observed for interferon(IFN)-γ production. When combined, these agonists primed MoDC to increase further the production of IFN-γ by CD4 + T cells in co-culture, especially those of naive (CD45RA +) phenotype, and IL-17 by memory (CD45RO +) CD4 + T cells. Conclusions. Ligation of TLR3 and Dectin-1 receptor up-regulates T-helper (Th) 1 and Th17 immune responses compared with single agonists. These findings may have therapeutic implications for the use of MoDC in immunotherapy. © 2012 Informa Healthcare.en_US
dc.language.isoenen_US
dc.relation.ispartofCytotherapyen_US
dc.subjectDectin-1 receptoren_US
dc.subjectHuman monocyte-derived dendritic cellsen_US
dc.subjectT-helper immune responseen_US
dc.subjectToll-like receptor 3en_US
dc.titleSignaling through Toll-like receptor 3 and Dectin-1 potentiates the capability of human monocyte-derived dendritic cells to promote T-helper 1 and T-helper 17 immune responsesen_US
dc.typeArticleen_US
dc.identifier.doi10.3109/14653249.2012.667873-
dc.identifier.pmid22424215-
dc.identifier.scopus2-s2.0-84859745341-
dc.identifier.urlhttps://api.elsevier.com/content/abstract/scopus_id/84859745341-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.openairetypeArticle-
item.fulltextWith Fulltext-
item.grantfulltextrestricted-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.author.deptChair of General Physiology and Biophysics-
crisitem.author.orcid0000-0002-1238-1731-
Appears in Collections:Journal Article
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