Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/1191
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dc.contributor.authorManojlovic, Dragicaen_US
dc.contributor.authorDramićanin, Miroslav D.en_US
dc.contributor.authorMiletic, Vesnaen_US
dc.contributor.authorMitić Ćulafić, Draganaen_US
dc.contributor.authorJovanović, Bojanaen_US
dc.contributor.authorNikolić, Biljanaen_US
dc.date.accessioned2019-08-27T11:12:22Z-
dc.date.available2019-08-27T11:12:22Z-
dc.date.issued2017-04-01-
dc.identifier.issn0109-5641-
dc.identifier.urihttps://biore.bio.bg.ac.rs/handle/123456789/1191-
dc.description.abstract© 2017 The Academy of Dental Materials Objective To compare cytotoxicity and genotoxicity of novel urethane-based monomer FIT-852 and monoacylphosphine oxide photoinitiator (Lucirin TPO) with conventional Bisphenol A-glycidyl-methacrylate (BisGMA) and triethylene glycol dimethacrylate (TEGDMA) monomers and camphorquinone (CQ)/amine photoinitiator system, respectively. Moreover, we quantified and analyzed the combinatorial effects of individual substances in resin-based mixtures concerning the nature of the combinatorial effects. Methods Cytotoxic and genotoxic effects of BisGMA, FIT, TEGDMA, CQ, DMAEMA and TPO and their combined toxicity in four clinically relevant mixtures (FIT/TPO, FIT/CQ, BisGMA/TPO, BisGMA/CQ) were tested on human fetal lung fibroblasts MRC-5 using MTT and Comet assays. We assessed combination effects of monomers and photoinitiators on overall toxicity from the measured concentration-effect relationships. Combination index (CI) was calculated on the basis of the median-effect equation derived from the mass-action law principle. Results Individual substances showed decreasing cytotoxic effects in the following order: BisGMA>TPO>FIT>CQ>DMAEMA>TEGDMA. Experimental mixtures showed decreasing cytotoxic effects in the order BisGMA/TPO>BisGMA/CQ>FIT/CQ>FIT/TPO. FIT-based mixtures exhibited antagonistic cytotoxic effects between components while BisGMA-based mixtures demonstrated synergistic effects at ED50. TPO amplified both antagonistic and synergistic cytotoxic effects in mixtures. Pure substances showed genotoxicity in the following order: TPO>BisGMA>FIT>CQ>TEGDMA. We did not detect the genotoxic potential of DMAEMA. The rank of genotoxic concentrations of the mixtures was: BisGMA/TPO>BisGMA/CQ>FIT/CQ>FIT/TPO. Significance Lower cytotoxicity and genotoxicity of FIT than BisGMA suggests its greater biocompatibility. Conversely, photoinitiator TPO was significantly more cytotoxic and genotoxic than both CQ and DMAEMA. CI values showed that components of FIT-based mixtures exhibit an antagonistic cytotoxic effect, while compontents of BisGMA-based mixtures show synergism.en_US
dc.language.isoenen_US
dc.relation.ispartofDental Materialsen_US
dc.subjectCombinatorial effecten_US
dc.subjectPhotoinitiatoren_US
dc.subjectMonomeren_US
dc.subjectGenotoxicityen_US
dc.subjectCytotoxicityen_US
dc.titleCytotoxicity and genotoxicity of a low-shrinkage monomer and monoacylphosphine oxide photoinitiator: Comparative analyses of individual toxicity and combination effects in mixturesen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.dental.2017.02.002-
dc.identifier.pmid28258769-
dc.identifier.scopus2-s2.0-85014043733-
dc.identifier.urlhttps://api.elsevier.com/content/abstract/scopus_id/85014043733-
item.fulltextNo Fulltext-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.author.deptChair of Microbiology-
crisitem.author.deptChair of Microbiology-
crisitem.author.orcid0000-0002-6651-6814-
crisitem.author.orcid0000-0003-1765-2454-
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