Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/1180
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dc.contributor.authorVasilijević, A.en_US
dc.contributor.authorVojčić, Ljen_US
dc.contributor.authorDinulović, I.en_US
dc.contributor.authorBuzadžić, B.en_US
dc.contributor.authorKorać, Aleksandraen_US
dc.contributor.authorPetrović, V.en_US
dc.contributor.authorJanković, A.en_US
dc.contributor.authorKorać, Batoen_US
dc.date.accessioned2019-08-16T12:55:39Z-
dc.date.available2019-08-16T12:55:39Z-
dc.date.issued2010-08-01-
dc.identifier.issn1089-8603-
dc.identifier.urihttps://biore.bio.bg.ac.rs/handle/123456789/1180-
dc.description.abstractMetabolic abnormalities underlying diabetes can be abrogated by l-arginine. Here we examined the molecular basis of disturbed interscapular brown adipose tissue (IBAT) thermogenesis and the possible role of nitric oxide (NO) in the IBAT of diabetic rats. To induce diabetes, adult Mill Hill hybrid hooded male rats were given a single alloxan dose (120 mg/kg). Both non-diabetic and diabetic groups were further divided into three subgroups receiving: (i) l-arginine·HCl (2.25%) or (ii) Nω-nitro-l-arginine methyl ester (l-NAME·HCl, 0.01%) for 12 days in drinking water and (iii) untreated controls. Treatment of the diabetic animals started after diabetes induction (glucose level ≥12 mmol/L). Diabetes led to a decrease in the mRNA levels of uncoupling protein 1 (UCP1), peroxisomal proliferator activator receptor gamma (PPARγ) and endothelial NO synthase (eNOS) as revealed by RT-PCR. The diabetic rats had reduced eNOS and inducible NOS (iNOS) protein contents accompanied by low tissue vascularization, a parameter directly related to tissue thermogenic state. Downregulation of glutathione peroxidase (GSH-Px) and catalase (CAT) transcripts were also observed in diabetes. In contrast, the expression level of PPARγ coactivator-1α (PGC-1α) mRNA was elevated. Supplementation with l-arginine not only restored diabetes-induced changes in the expressions of these molecules important for IBAT regulation, but also increased the vascularity. Interestingly, l-NAME induced similar patterns of changes in vascularity and PGC-1α mRNA level as did l-arginine. In summary, our results provide insight into the molecular basis underlying diabetes-induced metabolic and functional disturbances in the IBAT and suggest a beneficial role for the l-arginine-NO production pathway. © 2010 Elsevier Inc. All rights reserved.en_US
dc.language.isoenen_US
dc.relationMinistry of Science and Technological Development of the Republic of Serbia [143050]en_US
dc.relationCOST B35 Actionen_US
dc.relation.ispartofNitric Oxide - Biology and Chemistryen_US
dc.subjectDiabetes mellitusen_US
dc.subjectInterscapular brown adipose tissueen_US
dc.subjectL-Arginineen_US
dc.subjectNitric oxideen_US
dc.subjectThermogenesisen_US
dc.titleExpression pattern of thermogenesis-related factors in interscapular brown adipose tissue of alloxan-treated rats: Beneficial effect of l-arginineen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.niox.2010.04.001-
dc.identifier.pmid20403454-
dc.identifier.scopus2-s2.0-77952287533-
dc.identifier.urlhttps://api.elsevier.com/content/abstract/scopus_id/77952287533-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.openairetypeArticle-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.author.deptChair of Cell and Tissue Biology-
crisitem.author.orcid0000-0002-3044-9963-
crisitem.author.orcid0000-0001-5272-579X-
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