Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/1168
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dc.contributor.authorČvoro, Aleksandraen_US
dc.contributor.authorBajić, Aleksandaren_US
dc.contributor.authorZhang, Aijunen_US
dc.contributor.authorSimon, Marisaen_US
dc.contributor.authorGolić, Igoren_US
dc.contributor.authorSieglaff, Douglas H.en_US
dc.contributor.authorMaletic-Savatic, Mirjanaen_US
dc.contributor.authorKorać, Aleksandraen_US
dc.contributor.authorWebb, Paulen_US
dc.date.accessioned2019-08-16T12:00:35Z-
dc.date.available2019-08-16T12:00:35Z-
dc.date.issued2016-10-01-
dc.identifier.urihttps://biore.bio.bg.ac.rs/handle/123456789/1168-
dc.description.abstractThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Thyroid hormone (TH) receptors (TRs α and β) are homologous ligand-dependent transcriptio factors (TFs). While the TRs display distinct actions in development, metaboli regulation and other processes, comparisons of TRα and TRβ dependent gene regulatio mostly reveal similar mechanisms of action and few TR subtype specific genes. Here, w show that TRα predominates in multipotent human adipose derived stem cells (hADSC whereas TRβ is expressed at lower levels and is upregulated during hADSC differentiation The TRs display several unusual properties in parental hADSC. First, TRs display predominantl cytoplasmic intracellular distribution and major TRα variants TRα1 and TRα2 colocaliz with mitochondria. Second, knockdown experiments reveal that endogenous TR influence hADSC cell morphology and expression of hundreds of genes in the absence o hormone, but do not respond to exogenous TH. Third, TRα and TRβ affect hADSC i completely distinct ways; TRα regulates cell cycle associated processes while TRβ ma repress aspects of differentiation. TRα splice variant specific knockdown reveals that TRα and TRα2 both contribute to TRα-dependent gene expression in a gene specific manner We propose that TRs work in a non-canonical and hormone independent manner i hADSC and that prominent subtype-specific activities emerge in the context of thes unusual actions.en_US
dc.description.sponsorshipCenter for Electron Microscopy at Faculty of Biology, University of Belgradeen_US
dc.language.isoenen_US
dc.relationNIH [U01 GM094614, P30 A1036211, P30 CM 25123, S10 RR024574]en_US
dc.relationNICHD [1U54 HD083092]en_US
dc.relation.ispartofPLoS ONEen_US
dc.titleLigand independent and subtype-selective actions of thyroid hormone receptors in human adipose derived stem cellsen_US
dc.typeArticleen_US
dc.identifier.doi10.1371/journal.pone.0164407-
dc.identifier.pmid27732649-
dc.identifier.scopus2-s2.0-84991511087-
dc.identifier.urlhttps://api.elsevier.com/content/abstract/scopus_id/84991511087-
dc.description.rankM21en_US
dc.description.impact3.234en_US
dc.description.startpagee0164407en_US
dc.relation.issn1932-6203en_US
dc.description.volume11en_US
dc.description.issue10en_US
item.languageiso639-1en-
item.cerifentitytypePublications-
item.openairetypeArticle-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.author.deptChair of Cell and Tissue Biology-
crisitem.author.deptChair of Cell and Tissue Biology-
crisitem.author.deptChair of Cell and Tissue Biology-
crisitem.author.orcid0009-0007-5643-1634-
crisitem.author.orcid0000-0001-5944-5053-
crisitem.author.orcid0000-0002-3044-9963-
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