Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/1037
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dc.contributor.authorLaketa, Danijelaen_US
dc.contributor.authorSavić, Jasminaen_US
dc.contributor.authorBjelobaba, Ivanaen_US
dc.contributor.authorLavrnja, Irenaen_US
dc.contributor.authorVasić, Vesnaen_US
dc.contributor.authorStojiljković, Mirjanaen_US
dc.contributor.authorNedeljković, Nadeždaen_US
dc.date.accessioned2019-07-22T12:16:50Z-
dc.date.available2019-07-22T12:16:50Z-
dc.date.issued2015-01-01-
dc.identifier.issn1452-8258-
dc.identifier.urihttps://biore.bio.bg.ac.rs/handle/123456789/1037-
dc.description.abstract© by Danijela Laketa 2015. Background: Cortical stab injury (CSI) induces changes in the activity, expression and cellular distribution of specific ectonucleotidases at the injury site. Also, several experimentally induced neuropathologies are associated with changes in soluble ectonucleotidase activities in the plasma and serum, whilst various insults to the brain alter purine compounds levels in cerebrospinal fluid, but also in serum, indicating that insults to the brain may induce alterations in nucleotides release and rate of their hydrolysis in the vascular system. Since adenine nucleotides and adenosine regulate diverse cellular functions in the vascular system, including vascular tone, platelet aggregation and inflammatory responses of lymphocytes and macrophages, alterations of ectonucleotidase activities in the vascular system may be relevant for the clinical outcome of the primary insult. Methods: We explored ectonucleotidase activities using specific enzyme assays and determined adenine nucleotides concentrations by the UPLC method in the rat serum after cortical stab injury. while phosphodiesterase activity remained unchanged. Also, at 4-h postinjury a marked decrease in ATP concentration and more than 2-fold increase in AMP concentration were recorded. Conclusions: CSI induces rapid up-regulation of nucleotide catabolizing soluble ectonucleotidases in rat serum, which leads to the observed shift in serum nucleotide levels. The results obtained imply that ectonucleotidases and adenine nucleotides participate in the communication between the brain and the vascular system in physiological and pathological conditions and thereby may be involved in the development of various human neuropathologies.en_US
dc.language.isoenen_US
dc.relation.ispartofJournal of Medical Biochemistryen_US
dc.subjectATPen_US
dc.subjectbrain injuryen_US
dc.subjectectonucleotidasesen_US
dc.subjectextracellular adenine nucleo tidesen_US
dc.subjectrat serumen_US
dc.titleBrain injury alters ectonucleotidase activities and adenine nucleotide levels in rat serumen_US
dc.typeArticleen_US
dc.identifier.doi10.2478/jomb-2014-0025-
dc.identifier.pmid28356834-
dc.identifier.scopus2-s2.0-84925633777-
dc.identifier.urlhttps://api.elsevier.com/content/abstract/scopus_id/84925633777-
item.cerifentitytypePublications-
item.grantfulltextrestricted-
item.openairetypeArticle-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
crisitem.author.deptChair of General Physiology and Biophysics-
crisitem.author.deptChair of General Physiology and Biophysics-
crisitem.author.orcid0000-0002-6563-8924-
crisitem.author.orcid0000-0003-3046-0983-
Appears in Collections:Journal Article
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