Articles in peer-reviewed publications that disseminate the results of original research and scholarship. https://biore.bio.bg.ac.rs/handle/123456789/2 2026-11-10T09:30:55Z 2026-11-10T09:30:55Z Mićović, T. Samardžić, S. Veličković, Ivona Mileski, Ksenija Đorović Jovanović, I. https://biore.bio.bg.ac.rs/handle/123456789/8504 2026-11-09T12:26:20Z 2026-01-01T00:00:00Z

Title: Phytochemical characterization, antioxidant potential and multifunctional enzyme inhibitory activity of Hyssopus officinalis subsp. aristatus: An in vitro and in silico study Authors: Mićović, T.; Samardžić, S.; Veličković, Ivona; Mileski, Ksenija; Đorović Jovanović, I. Abstract: LC-DAD-ESI-MS analysis of the methanol extract from the flowering aerial parts of Hyssopus officinalis subsp. aristatus (Montenegro) revealed the predominance of hydroxycinnamic acid derivatives, flavonoid glycosides, and phenylethanoid glycosides. The extract exhibited moderate antioxidant activity in ABTS, TRC, and BCB assays, with the strongest effect in ABTS (IC50 = 73.41 µg/mL), compared to L-ascorbic acid and BHT (31.59 and 40.37 µg/mL, respectively). Major phenolics, chlorogenic and rosmarinic acids, showed potent antioxidant activity (IC50 = 24.00 and 12.23 µg/mL in the ABTS assay, respectively). The extract showed stronger inhibition of α-glucosidase (IC50 = 19.02 mg/mL) than α-amylase (26.41 mg/mL). The dominant phenolic acids showed potent α-glucosidase inhibition, comparable to acarbose. This study is the first to report inhibitory activity of this species against arginase (IC50 = 7.47 mg/mL) and elastase (IC50 = 26.24 mg/mL). Molecular docking indicated favorable binding of rosmarinic acid to α-glucosidase (ΔGbind = -7.56 kcal/mol) and arginase (ΔGbind = -7.77 kcal/mol), providing a theoretical rationale for the extract's activity. These findings highlight this plant as a promising source of multifunctional phenolics relevant to oxidative stress and metabolic disorders.

2026-01-01T00:00:00Z Džamić, Ana M. Matejić, Jelena S. Mileski, Ksenija Žarković, Lazar D. Marković, Marija S. Dragićević, Anđela V. Gašić, Uroš M. Mišić, Danijela M. Ćirić, Ana D. https://biore.bio.bg.ac.rs/handle/123456789/8503 2026-11-09T12:23:20Z 2026-08-01T00:00:00Z

Title: Comparative Phytochemical Characterization and Bioactivity Profiling of Serbian Ajuga Species from Different Localities: Antioxidant, Antityrosinase, Antimicrobial and Anti-Pyocyanin Activities Authors: Džamić, Ana M.; Matejić, Jelena S.; Mileski, Ksenija; Žarković, Lazar D.; Marković, Marija S.; Dragićević, Anđela V.; Gašić, Uroš M.; Mišić, Danijela M.; Ćirić, Ana D. Abstract: Herbaceous and subshrub species of the genus Ajuga are recognized as valuable sources of bioactive metabolites with diverse pharmacological properties. This study comparatively evaluated the phytochemical composition and the in vitro biological activities of Ajuga chamaepitys, A. genevensis, A. laxmannii, and A. reptans collected from different localities in Serbia. UHPLC-MS/MS profiling identified p-hydroxybenzoic acid and ferulic acid as the predominant phenolic compounds. A. reptans exhibited the highest total phenolic content (120.52 mg GAE/g dw), along with the strongest antioxidant activity in DPPH, ABTS, β-carotene bleaching, and FRAP assays, as well as the highest antibacterial activity. A. laxmannii contained the highest flavonoid content (48.95 mg QuE/g dw) and showed the strongest antifungal activity, whereas A. genevensis demonstrated the most potent tyrosinase inhibitory activity. Notably, A. genevensis extracts exhibited pronounced anti-pyocyanin activity, approximately twofold stronger than ampicillin. These findings demonstrate significant species- and locality-dependent variation in the phytochemical composition and biological activity and identify Serbian Ajuga species as promising natural sources of antioxidant, antimicrobial, and antivirulence compounds with potential pharmaceutical and food applications.

2026-08-01T00:00:00Z Vujović, Predrag https://biore.bio.bg.ac.rs/handle/123456789/8499 2026-11-09T11:38:33Z 2026-09-01T00:00:00Z

Title: The missing chapter of physiology: the importance of teaching the physiology of dying Authors: Vujović, Predrag Abstract: NEW & NOTEWORTHY Physiology education rarely addresses the biological processes that occur at the end of life. This article proposes that teaching the physiology of dying provides a powerful integrative framework for reinforcing core physiological con cepts such as homeostasis, energy use, and systems integration. Using interactive teaching and pattern recognition-based learn ing in an undergraduate physiology course, we demonstrate how the physiology of dying can help students consolidate conceptual understanding while completing the physiological narrative of the human lifespan.

2026-09-01T00:00:00Z Herrera, Jovita Vujović, Predrag https://biore.bio.bg.ac.rs/handle/123456789/8498 2026-11-09T10:58:33Z 2026-03-07T00:00:00Z

Title: Rethinking physiology teaching: evidence and theory-informed strategies for interactive physiology education Authors: Herrera, Jovita; Vujović, Predrag Abstract: NEW & NOTEWORTHY Unlike broad, older “active learning” overviews, this review applies a physiology-specific, Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) 2020-guided approach [protocol registered on Open Science Framework (OSF)] that integrates empirical evidence with theory to produce actionable guidance. It updates the field through an exhaustive 1990 to Aug 2025 search and quality appraisal [Medical Education Research Study Quality Instrument (MERSQI)/Mixed Methods Appraisal Tool (MMAT)] and then maps findings onto six persistent, discipline-relevant challenges (large-class engagement; abstract/paired concepts; fragmented knowledge; system-level complexity; preclass preparation; and formative feedback). Beyond cataloging strategies, it links each challenge to concrete, small-step interventions (interactive lecturing with polling, peer instruction, scaffolded problem-solving, pattern recognition, system-based frameworks for acid-base, flipped elements, and low-stakes assessment) explicitly grounded in learning theory (constructivism, cognitive load, dual coding, and retrieval practice). Sensitivity analyses demonstrate thematic robustness. The result is a practical, evidence-and-theory-informed toolkit that shows educators how to incrementally transform physiology teaching without curricular overhaul.

2026-03-07T00:00:00Z