https://biore.bio.bg.ac.rs/handle/123456789/1 2026-11-10T09:27:09Z 2026-11-10T09:27:09Z Mićović, T. Samardžić, S. Veličković, Ivona Mileski, Ksenija Đorović Jovanović, I. https://biore.bio.bg.ac.rs/handle/123456789/8504 2026-11-09T12:26:20Z 2026-01-01T00:00:00Z

Title: Phytochemical characterization, antioxidant potential and multifunctional enzyme inhibitory activity of Hyssopus officinalis subsp. aristatus: An in vitro and in silico study Authors: Mićović, T.; Samardžić, S.; Veličković, Ivona; Mileski, Ksenija; Đorović Jovanović, I. Abstract: LC-DAD-ESI-MS analysis of the methanol extract from the flowering aerial parts of Hyssopus officinalis subsp. aristatus (Montenegro) revealed the predominance of hydroxycinnamic acid derivatives, flavonoid glycosides, and phenylethanoid glycosides. The extract exhibited moderate antioxidant activity in ABTS, TRC, and BCB assays, with the strongest effect in ABTS (IC50 = 73.41 µg/mL), compared to L-ascorbic acid and BHT (31.59 and 40.37 µg/mL, respectively). Major phenolics, chlorogenic and rosmarinic acids, showed potent antioxidant activity (IC50 = 24.00 and 12.23 µg/mL in the ABTS assay, respectively). The extract showed stronger inhibition of α-glucosidase (IC50 = 19.02 mg/mL) than α-amylase (26.41 mg/mL). The dominant phenolic acids showed potent α-glucosidase inhibition, comparable to acarbose. This study is the first to report inhibitory activity of this species against arginase (IC50 = 7.47 mg/mL) and elastase (IC50 = 26.24 mg/mL). Molecular docking indicated favorable binding of rosmarinic acid to α-glucosidase (ΔGbind = -7.56 kcal/mol) and arginase (ΔGbind = -7.77 kcal/mol), providing a theoretical rationale for the extract's activity. These findings highlight this plant as a promising source of multifunctional phenolics relevant to oxidative stress and metabolic disorders.

2026-01-01T00:00:00Z Džamić, Ana M. Matejić, Jelena S. Mileski, Ksenija Žarković, Lazar D. Marković, Marija S. Dragićević, Anđela V. Gašić, Uroš M. Mišić, Danijela M. Ćirić, Ana D. https://biore.bio.bg.ac.rs/handle/123456789/8503 2026-11-09T12:23:20Z 2026-08-01T00:00:00Z

Title: Comparative Phytochemical Characterization and Bioactivity Profiling of Serbian Ajuga Species from Different Localities: Antioxidant, Antityrosinase, Antimicrobial and Anti-Pyocyanin Activities Authors: Džamić, Ana M.; Matejić, Jelena S.; Mileski, Ksenija; Žarković, Lazar D.; Marković, Marija S.; Dragićević, Anđela V.; Gašić, Uroš M.; Mišić, Danijela M.; Ćirić, Ana D. Abstract: Herbaceous and subshrub species of the genus Ajuga are recognized as valuable sources of bioactive metabolites with diverse pharmacological properties. This study comparatively evaluated the phytochemical composition and the in vitro biological activities of Ajuga chamaepitys, A. genevensis, A. laxmannii, and A. reptans collected from different localities in Serbia. UHPLC-MS/MS profiling identified p-hydroxybenzoic acid and ferulic acid as the predominant phenolic compounds. A. reptans exhibited the highest total phenolic content (120.52 mg GAE/g dw), along with the strongest antioxidant activity in DPPH, ABTS, β-carotene bleaching, and FRAP assays, as well as the highest antibacterial activity. A. laxmannii contained the highest flavonoid content (48.95 mg QuE/g dw) and showed the strongest antifungal activity, whereas A. genevensis demonstrated the most potent tyrosinase inhibitory activity. Notably, A. genevensis extracts exhibited pronounced anti-pyocyanin activity, approximately twofold stronger than ampicillin. These findings demonstrate significant species- and locality-dependent variation in the phytochemical composition and biological activity and identify Serbian Ajuga species as promising natural sources of antioxidant, antimicrobial, and antivirulence compounds with potential pharmaceutical and food applications.

2026-08-01T00:00:00Z Mileski, Ksenija Samardžić, S. Arsenijević, J. Janković, S. Veličković, Ivona https://biore.bio.bg.ac.rs/handle/123456789/8502 2026-11-09T12:21:03Z 2026-09-16T00:00:00Z

Title: Inhibitorno dejstvo taninske i netaninske frakcije tropa kupine sorte „Čačanska bestrna“ na digestivne enzime Authors: Mileski, Ksenija; Samardžić, S.; Arsenijević, J.; Janković, S.; Veličković, Ivona Description: Knjiga sažetaka: Str.125

2026-09-16T00:00:00Z Mileski, Ksenija Samardžić, S. Arsenijević, J. Janković, S. Veličković, Ivona https://biore.bio.bg.ac.rs/handle/123456789/8501 2026-11-09T12:18:46Z 2026-09-16T00:00:00Z

Title: Antiradikalski i redukcioni efekti frakcija dobijenih iz tropa kupine sorte „Čačanska bestrna“ Authors: Mileski, Ksenija; Samardžić, S.; Arsenijević, J.; Janković, S.; Veličković, Ivona Description: Knjiga sažetaka: Str. 124

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